Differences betwixt Gram positive bacteria together with Gram negative bacteria Gram positive bacteria Gram negative bacteria 1. The bacteria stay coloured alongside Gram staining fifty-fifty later on washing alongside alcohol or acetone The bacteria create non retain the stain when washed alongside alcohol or acetone. So these type of bacteria are observed alongside roughly other stain such equally safranin. 2. Outer membrane is absent Present 3. Thicker jail cellular telephone wall. Thickness varies from 20-80 nm Thin jail cellular telephone wall. Thickness varies from 8-12 nm 4. Smooth wall Wavy wall together with comes inwards contact alongside plasma membrane exclusively at a few loci. 5. The wall contains 70-80% peptidoglycan ...